Genetic structure and connectivity among Aedes aegypti populations within Madurai city in Southern India

We investigated the genetic variability and differentiation among 12 Ae. aegypti populations collected within the Madurai city in Tamil Nadu state of Southern India. Genotyping of 12 microsatellite markers in 353 individual samples showed moderate levels of genetic diversity among 12 populations. UPGMA tree, hierarchical clustering, Bayesian clustering and Discriminant Analysis on Principal Components roughly divided these populations into two genetic clusters: main city populations and the populations located at the border of the corporation limit. Significant positive correlation between genetic and geographic distance was observed among 12 populations, however, the correlation was non-significant within each genetic cluster. Population assignment and divMigrate graph depicted less migration between two groups. Overall, the findings of this study provided an overview of Ae. aegypti population structure within an urban setting in India that have implications in effective implementation of vector control in the city area. •Fine scale genetic structure of Aedes aegypti within a city.•Genetic structure roughly associated with geographical location.•Main city populations were found less connected with the border populations.•Genetic and geographic distance was positively correlated among all the populations.•However, the correlation was non-significant among main city populations.