A bifunctional probe reveals increased viscosity and hydrogen sulfide in zebra fish model of Parkinson's disease

Altered H2S levels and intracellular viscosity have both been seen in Parkinson's disease (PD). However, how H2S and intracellular viscosity are involved in PD pathogenesis remains unknown. Herein, a dual-function fluorescent probe DF was designed and synthesized to analyze intracellular viscosity and hydrogen sulfide. It is a near-infrared fluorescence probe with improved photostability and large Stokes shift (110 nm). The probe reveals increased viscosity and hydrogen sulfide in zebrafish model of PD for the first time. [Display omitted] •A bifunction fluorescent probe DF could selectively detect viscosity and hydrogen sulfide in the whole cells.•The probe was successfully applied in vivo and in vitro to detect the alteration of viscosity and H2S.•The probe reveals increased viscosity and hydrogen sulfide in zebrafish models of Parkinson's disease for the first time.