Growth at 5 kPa Causes Differential Expression of a Number of Signals in a Bacillus subtilis Strain Adapted to Enhanced Growth at Low Pressure

To determine microbial evolutionary strategies to low-pressure (LP; 5 kPa) growth, an environmental condition not experienced on Earth until ∼20 km in altitude, a previously described evolutionary experiment was conducted. The resulting LP evolved strain WN1106, isolated from the terminus of the experiment, was shown to have several genomic mutations absent in the ancestral strain, WN624. Three of the mutations were in regulatory genes: resD, walK, and rnjB. Here we report on transcriptional microarray data from the LP-evolved WN1106 and compare those results with the previously reported ancestral WN624 transcriptional array data at either 5 or 101 kPa. At 5 kPa, WN1106 differentially expresses signals that are under the control of regulators ResD, WalK, and RnjB compared with (1) itself at ∼101 kPa and (2) WN624 at 5 kPa. These results were further confirmed by quantitative reverse transcriptase–polymerase chain reaction of a target transcript from each regulon. This work indicates that the three mutated coding regions had transcriptional control effects on each respective regulon.