Steroidobacter gossypii sp. nov., isolated from cotton field soil

A Gram-negative bacterium, designated S1-65 , was isolated from soil samples collected from a cotton field located in the Xinjiang region of PR China. Results of 16S rRNA gene sequence analysis revealed that strain S1-65 was affiliated to the genus with its closest phylogenetic relatives being ' ' 35Y (98.4 %), ' ' SA29-B (98.3 %) and KA5-B (98.3 %). 16S rRNA-directed phylogenetic analysis showed that strain S1-65 formed a unique phylogenetic subclade next to ' ' SA29-B and KA5-B , suggesting that strain S1-65 should be identified as a member of the genus . Further, substantial differences between the genotypic properties of strain S1-65 and the members of the genus , including average nucleotide identity and digital DNA-DNA hybridization, resolved the taxonomic position of strain S1-65 and suggested its positioning as representing a novel species of the genus . The DNA G+C content of strain S1-65 was 62.5 mol%, based on its draft genome sequence. The predominant respiratory quinone was ubiquinone-8. The main fatty acids were identified as summed feature 3 (C ω6 /C ω7 ), C and iso-C . In addition, its polar lipid profile was composed of aminophospholipid, diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Here, we propose a novel species of the genus : sp. nov. with the type strain S1-65 (=JCM 34287 =CGMCC 1.18736 ).