Hymenobacter puniceus sp. nov., radiation resistant bacteria isolated from soil in South Korea

Two novel Gram-negative bacterial strains BT190 T and BT191 were isolated from soil collected in Uijeongbu city (37°44′55″N, 127°02′20″E), Korea. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strains BT190 T and BT191 belong to a distinct lineage within the genus Hymenobacter (family Hymenobacteraceae , order Cytophagales , class Cytophagia ). The level of 16S rRNA gene sequence similarity between the strains BT190 T and BT190 was 99.5%. The strains BT190 T and BT191 were closely related to Hymenobacter swuensis DY53 T (97.0% 16S rRNA gene similarity), Hymenobacter metallilatus 9PBR-2 T (96.8%), Hymenobacter tibetensis XTM003 T (96.8%) and Hymenobacter yonginensis HMD1010 T (96.6%). The genome size of strain BT190 T was 4,859,864 bp. The DNA G+C content of strain BT190 T was 55.3 mol%. Bacterial growth was observed at 4–30 °C (optimum 25 °C) and pH 6.0–9.0 (optimum, pH 6.0–7.0) on R2A agar. Colonies of strains BT190 T and BT191 were raised, smooth, circular and red-pink colored. The sole respiratory quinone of strain BT190 T was MK-7 and the predominant cellular fatty acids were iso-C 15:0 , C 16:1 ω 5 c , summed feature 3 (C 16:1 ω 6 c / C 16:1 ω 7 c ) and summed feature 4 (iso-C 17:1 I / anteiso-C 17:1 B). The major polar lipids of strain BT190 T were aminophospholipid (APL) and phosphatidylethanolamine (PE). Based on the chemotaxonomic, biochemical, and phylogenetic analysis, strains BT190 T and BT191 can be suggested as a novel bacterial species within the genus Hymenobacter, for which the name Hymenobacter puniceus sp. nov is proposed . The type strain of Hymenobacter puniceus is BT190 T (= KCTC 72342 T = NBRC 114860 T ).