Hepatitis E Virus Persistence and/or Replication in the Peripheral Blood Mononuclear Cells of Acute HEV-Infected Patients

Hepatitis E Virus Persistence and/or Replication in the Peripheral Blood Mononuclear Cells of Acute HEV-Infected Patients

Flow of the study design: Blood collected from acute HEV patients were fractiontated into plasma and PBMCs. Plasma was used for cytokine assay. RNA was extracted from the PBMCs and used for determinaton of viral load, detection of (+) and (−) HEV RNA strands, and gene expression assay.

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Veröffentlicht in:Frontiers in microbiology 2021-07, Vol.12, p.696680-696680
Hauptverfasser: Sayed, Ibrahim M., Abd Elhameed, Zeinab A., Abd El-Kareem, Doaa M., Abdel-Malek, Mohamed A. Y., Ali, Mohamed E., Ibrahim, Maggie A., Sayed, Ayat Abdel-Rahman, Khalaf, Khaled Abo bakr, Abdel-Wahid, Lobna, El-Mokhtar, Mohamed A.
Format: Artikel
Sprache:eng
Schlagworte:
acute hepatitis
cytokines
gene expression
HEV
Microbiology
PBMCs
persistence and replication
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Beschreibung
Zusammenfassung:Flow of the study design: Blood collected from acute HEV patients were fractiontated into plasma and PBMCs. Plasma was used for cytokine assay. RNA was extracted from the PBMCs and used for determinaton of viral load, detection of (+) and (−) HEV RNA strands, and gene expression assay.
ISSN:1664-302X
1664-302X
DOI:10.3389/fmicb.2021.696680