Mariniflexile maritimum sp. nov., isolated from seawater of the South Sea in the Republic of Korea

A novel Gram-stain-negative, rod-shaped, aerobic, non-motile bacterial strain, designated M5A1M T , was isolated from seawater collected from the South Sea of the Republic of Korea. Based on 16S rRNA gene sequence similarity, strain M5A1M T was closely related to Mariniflexile gromovii KMM 6038 T (95.3 %), Mariniflexile fucanivorans SW5 T (95.2 %), Mariniflexile soesokkakense RSSK-9 T (95.1 %), Yeosuana aromativorans GW1-1 T (94.6 %) and Confluentibacter lentus HJM-3 T (94.6 %). Genome-based phylogenetic analyses revealed that strain M5A1M T formed a distinct cluster with the type strains of the genus Mariniflexile . The major cellular fatty acid constituents (>5 % of the total fatty acids) were iso-C 15:0 , anteiso-C 15 : 0 , iso-C 15 : 0 3-OH, iso-C 15 : 1 G, iso-C 16:0 3-OH and iso-C 17 : 0 3-OH. The respiratory quinone was identified as MK-6. The major polar lipids were phosphatidylethanolamine and one unidentified polar lipid. The genomic DNA G+C content of strain M5A1M T was determined to be 37.7 mol%. On the basis of its phenotypic, phylogenetic and chemotaxonomic characteristics, strain M5A1M T is considered to represent a novel species within the genus Mariniflexile , for which the name Mariniflexile maritimum sp. nov. is proposed. The type strain is M5A1M T (=KCTC 72895 T =JCM 33982 T ).