Study on the gelatin methacryloyl composite scaffold with exogenous transforming growth factor β 1 to promote the repair of skull defects

OBJECTIVETo prepare a bone tissue engineering scaffold for repairing the skull defect of Sprague Dawley (SD) rats by combining exogenous transforming growth factor β 1 (TGF-β 1) with gelatin methacryloyl (GelMA) hydrogel. METHODSFirstly, GelMA hydrogel composite scaffolds containing exogenous TGF-β...

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Veröffentlicht in:Zhongguo xiu fu chong jian wai ke za zhi 2021-07, Vol.35 (7), p.904-912
Hauptverfasser: Liu, Xiangyu, Wang, Zhaodong, Xu, Chen, Guan, Jianzhong, Wei, Bangguo, Liu, Yajun
Format: Artikel
Sprache:chi
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Zusammenfassung:OBJECTIVETo prepare a bone tissue engineering scaffold for repairing the skull defect of Sprague Dawley (SD) rats by combining exogenous transforming growth factor β 1 (TGF-β 1) with gelatin methacryloyl (GelMA) hydrogel. METHODSFirstly, GelMA hydrogel composite scaffolds containing exogenous TGF-β 1 at concentrations of 0, 150, 300, 600, 900, and 1 200 ng/mL (set to groups A, B, C, D, E, and F, respectively) were prepared. Cell counting kit 8 (CCK-8) method was used to detect the effect of composite scaffold on the proliferation of bone marrow mesenchymal stem cells (BMSCs) in SD rats. ALP staining, alizarin red staining, osteocalcin (OCN) immunofluorescence staining, and Western blot were used to explore the effect of scaffolds on osteogenic differentiation of BMSCs, and the optimal concentration of TGF-β 1/GelMA scaffold was selected. Thirty-six 8-week-old SD rats were taken to prepare a 5 mm diameter skull bone defect model and randomly divided into 3 groups, namely the control group, the GelMA group, and
ISSN:1002-1892
DOI:10.7507/1002-1892.202102008