Chitosan grafted butein: A metal-free transducer for electrochemical genosensing of exosomal CD24

Metal-free cost-efficient biocompatible molecules are beneficial for opto-electrochemical bioassays. Herein, chitosan (CS) conjugated butein is prepared via graft polymerization. Structural integrity between radical active sites of CS and its probable conjugation routes with reactive OH group of butein during grafting were comprehensively studied using optical absorbance/emission property, NMR, FT-IR and XPS analysis. Fluorescence emission of CS-conjugated butein (CSB) was studied in dried flaky state as well as in drop casted form. Cyclic voltammetric study of CSB modified glassy carbon electrode exhibits 2e−/2H+ transfer reaction in phosphate buffered saline electrolyte following a surface-confined process with a correlation coefficient of 0.99. Unlike pristine butein, CSB modified electrode display a highly reversible redox behavior under various pH ranging from 4 to 9. For the proof-of-concept CSB-modified flexible screen printed electrodes were processed for electrochemical biosensing of exosomal CD24 specific nucleic acid at an ultralow sample concentration, promising for ovarian cancer diagnosis. [Display omitted] •Chitosan conjugated butein (CSB) has been developed via graft polymerization.•Chemical structure analysis suggests that NH2 group of CS involved in conjugation.•CSB exhibit fluorescence emission function useful for in vitro and in vivo assays.•CSB modified electrode enable reversible 2e−/2H+ transfer reaction at various pH.•Genosensor platform based on CSB electrodes suitable for ovarian cancer diagnosis.