Assessment of the effect of endocrine abnormalities on biomacromolecules and lipids by FT-IR and biochemical assays as biomarker of metabolites in early Polycystic ovary syndrome women
•Early phase Polycystic ovary syndrome is characterized by differentiation in their biomacromolecules and lipid structures independent of obesity and metabolic syndrome.•Higher absorbance of (CH2) and (CH3) functional groups in the Polycystic ovary syndrome patient’s spectrum were visible.•The peak...
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Veröffentlicht in: | Journal of pharmaceutical and biomedical analysis 2021-09, Vol.204, p.114250-114250, Article 114250 |
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Zusammenfassung: | •Early phase Polycystic ovary syndrome is characterized by differentiation in their biomacromolecules and lipid structures independent of obesity and metabolic syndrome.•Higher absorbance of (CH2) and (CH3) functional groups in the Polycystic ovary syndrome patient’s spectrum were visible.•The peak corresponding to a-form of DNA patch (PO2−) can be used as a marker for Polycystic ovary syndrome disease.•A ccorrelation was not found between luteinizing hormone level and proteins in Polycystic ovary syndrome patient’s samples, while there were in healthy subjects.•In polycystic ovary syndrome, a negative correlation between carbohydrates and fatty acids were visible, while in healthy subjects this correlation was not noticed.
Polycystic ovary syndrome (PCOS) is a common endocrinopathy associated with metabolic disturbances. Both in the development and improvement of the disease, the structure of phospholipids and proteins in the blood serum plays important role in the treatment of these disease. Herein, to investigate the metabolic process and the variations of biomacromolecules and lipids between young PCOS women and healthy subjects, biochemistry and Fourier Transform InfraRed spectroscopy (FTIR) methods, were used. Moreover, partial least squares regression (PLS) and Principal component analysis (PCA) to research differentiation of biomacromolecules, were performed. We obtained blood serum of of 100 individuals including 57 with PCOS and 43 healthy controls. The biochemical blood profile of PCOS women was presented by spectroscopic measurements, which is an analytical technique, as well as by laboratory indexes and oxidative stress status measurements. There was a significant structural differentiation between studied groups in the number of functional groups and biomolecules differentiation depending on the protein expression and oxidative stress status. Hence, FTIR spectroscopy and oxidative load can be effectively utilized as tools for classifying quantitative and qualitative changes of biomolecules in PCOS samples. PCOS samples did not correlate with luteinizing hormone (LH) level and proteins but had a negative correlation between carbohydrates and fatty acids, compared with control group. |
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ISSN: | 0731-7085 1873-264X |
DOI: | 10.1016/j.jpba.2021.114250 |