Genome-wide expression analysis reveal host genes involved in immediate-early infections of different sheeppox virus strains
•Transcriptomic analysis of LTCs at immediate early infection of SPPV WS & SPPV VS.•Most DEGs are involved in various cellular pathways at 0.5h of SPPV-VS infection.•RICTOR and STAT3 identified as top upstream regulators in SPPV-VS & WS, respectively.•qRT-PCR confirms the up-regulation of CD...
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Veröffentlicht in: | Gene 2021-10, Vol.801, p.145850-145850, Article 145850 |
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Sprache: | eng |
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Zusammenfassung: | •Transcriptomic analysis of LTCs at immediate early infection of SPPV WS & SPPV VS.•Most DEGs are involved in various cellular pathways at 0.5h of SPPV-VS infection.•RICTOR and STAT3 identified as top upstream regulators in SPPV-VS & WS, respectively.•qRT-PCR confirms the up-regulation of CD40, FAS, NFKB2 & RICTOR genes in infected LTCs.•Dysregulation of DEGs involve in viral entry, replication & host cell defense.
This study explored the transcriptome of lamb testis cells infected with sheeppox virus (SPPV) wild strain (WS) and vaccine strain (VS) at an immediate-early time. Most of the differentially expressed genes (DEGs) and differentially expressed highly connected (DEHC) gene network were found to be involved in SPPV-VS infection compared to SPPV-WS. Further, the signaling pathways were mostly involved in SPPV-VS infection than SPPV-WS. SPPV modulates the expression of several important host proteins such as CD40, FAS, ITGβ1, ITGα1, Pak1, Pak2, CD14, ILK leading to viral attachment and entry; immune-related DEGs such as MAPK, JNK, ERK, NFKB, IKB, PI3K, STAT which provide optimal cellular condition for early viral protein expression; and FOXO3, ATF, CDKNA1, TCF, SRF, BDNF which help in inducing apoptosis and MPTP, BAD and Tp53 inhibits apoptosis or cell death at the immediate-early time. The results captured the specific genes and enabled to understand distinct pathogenic mechanisms employed by VS and WS of SPPV. |
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ISSN: | 0378-1119 1879-0038 |
DOI: | 10.1016/j.gene.2021.145850 |