Genome-wide expression analysis reveal host genes involved in immediate-early infections of different sheeppox virus strains

•Transcriptomic analysis of LTCs at immediate early infection of SPPV WS & SPPV VS.•Most DEGs are involved in various cellular pathways at 0.5h of SPPV-VS infection.•RICTOR and STAT3 identified as top upstream regulators in SPPV-VS & WS, respectively.•qRT-PCR confirms the up-regulation of CD...

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Veröffentlicht in:Gene 2021-10, Vol.801, p.145850-145850, Article 145850
Hauptverfasser: Sonowal, Joyshikh, Lal Patel, Chhabi, Kumar Gandham, Ravi, Sajjanar, Basavaraj, Ishaq Nabi Khan, Raja, Ranjan Praharaj, Manas, Akram Malla, Waseem, Kumar, Deepak, Dev, Kapil, Barkathullah, N., Bharali, Krishna, Dubey, Amitesh, Lalita, D., Zafir, Insha, Mishra, B.P., Mishra, Bina
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Sprache:eng
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Zusammenfassung:•Transcriptomic analysis of LTCs at immediate early infection of SPPV WS & SPPV VS.•Most DEGs are involved in various cellular pathways at 0.5h of SPPV-VS infection.•RICTOR and STAT3 identified as top upstream regulators in SPPV-VS & WS, respectively.•qRT-PCR confirms the up-regulation of CD40, FAS, NFKB2 & RICTOR genes in infected LTCs.•Dysregulation of DEGs involve in viral entry, replication & host cell defense. This study explored the transcriptome of lamb testis cells infected with sheeppox virus (SPPV) wild strain (WS) and vaccine strain (VS) at an immediate-early time. Most of the differentially expressed genes (DEGs) and differentially expressed highly connected (DEHC) gene network were found to be involved in SPPV-VS infection compared to SPPV-WS. Further, the signaling pathways were mostly involved in SPPV-VS infection than SPPV-WS. SPPV modulates the expression of several important host proteins such as CD40, FAS, ITGβ1, ITGα1, Pak1, Pak2, CD14, ILK leading to viral attachment and entry; immune-related DEGs such as MAPK, JNK, ERK, NFKB, IKB, PI3K, STAT which provide optimal cellular condition for early viral protein expression; and FOXO3, ATF, CDKNA1, TCF, SRF, BDNF which help in inducing apoptosis and MPTP, BAD and Tp53 inhibits apoptosis or cell death at the immediate-early time. The results captured the specific genes and enabled to understand distinct pathogenic mechanisms employed by VS and WS of SPPV.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2021.145850