Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1

Two-Photon, Ratiometric, Quantitative Fluorescent Probe Reveals Fluctuation of Peroxynitrite Regulated by Arginase 1

Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively vis...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Analytical chemistry (Washington) 2021-07, Vol.93 (29), p.10090-10098
Hauptverfasser: Chen, Shiyu, Vurusaner, Beyza, Pena, Stephanie, Thu, Chu T, Mahal, Lara K, Fisher, Edward. A, Canary, James. W
Format: Artikel
Sprache:eng
Schlagworte:
Analytical chemistry
Arginase
Chemistry
fluorescent dyes
Fluorescent indicators
Peroxynitrite
Photons
Reactive oxygen species
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:Peroxynitrite, a transient reactive oxygen species (ROS), is believed to play a deleterious role in physiological processes. Herein, we report a two-photon ratiometric fluorescent probe that selectively reacts with peroxynitrite yielding a >200-fold change upon reaction. The probe effectively visualized fluctuations in peroxynitrite generation by arginase 1 in vivo and in vitro. This provides evidence that arginase 1 is a critical regulator of peroxynitrite.
ISSN:0003-2700
1520-6882
1520-6882
DOI:10.1021/acs.analchem.1c00911