Identifying Plasmopara viticola resistance Loci in grapevine (Vitis amurensis) via genotyping-by-sequencing-based QTL mapping

Downy mildew, caused by Plasmopara viticola, is a major disease that affects grapevines, and a few resistance (R) genes have been identified thus far. In order to identify R genes, we investigated F1 progeny from a cross between the downy mildew-resistant Vitis amurensis ‘Shuang Hong’ and the susceptible Vitis vinifera ‘Cabernet Sauvignon’. The P. viticola-resistance of the progeny varied continuously and was segregated as a quantitative trait. Genotyping-by-sequencing was used to construct linkage maps. The integrated map spanned 1898.09 cM and included 5603 single nucleotide polymorphisms on 19 linkage groups (LGs). Linkage analysis identified three quantitative trait loci (QTLs) for P. viticola resistance: 22 (Rpv22) on LG 02, Rpv23 on LG15, and Rpv24 on LG18. The phenotypic variance contributed by these three QTLs ranged from 15.9 to 30.0%. qRT-PCR analysis of R-gene expression in these QTLs revealed a CC-NBS-LRR disease resistance gene RPP8, two LRR receptor-like serine/threonine-protein kinases, a serine/threonine-protein kinase BLUS1, a glutathione peroxidase 8, an ethylene-responsive transcription factor ERF038, and a transcription factor bZIP11 were induced by P. viticola, and these genes may play important role in P. viticola response. [Display omitted] •The Plasmopara viticola resistance phenotype indicated that the resistance was caused by quantitative trait loci.•High-density genetic map spanned 1898.09 cM and contained 5603.00 SNPs in 19 Linkage groups.•Three QTLs were identified for Rpv22 on Chromosome 02, Rpv23 on Chromosome 15, and Rpv24 on Chromosome 18.•The transcription of a CC-NBS-LRR type gene and six other type genes in these loci were induced by Plasmopara viticola.