Mycoplasma maculosum and Mycoplasma spumans associated with fertility disorders in dogs from a Bernese Mountain dog kennel

•Mycoplasma maculosum and Mycoplasma spumans were detected by PCR in dogs.•After antibiotic treatment reproductive disorders disappeared.•Veterinarians not often consider Mycoplasma in the differential diagnosis of reproductive diseases. The aim of this short communication is to describe a case of subfertility and other anomalies associated with the presence of Mycoplasma spumans and Mycoplasma maculosum in a Bernese Mountain Dog kennel. After the arrival of two dogs from abroad, some fertility disorders, such as unsuccessful mating, pregnancy losses and abnormal sperm analysis results, were observed. Two consecutive samplings (vaginal swabs) of three and two bitches with problems, respectively, were performed and M. spumans and M. maculosum were identified by PCR and sequencing. After treatment for 15 days with doxycycline and 9 days with azithromycin, successful pregnancies were achieved and the results of the sperm analyses were reversed. Considering that no other infectious agents causing subfertility problems were detected and that no management measures or other medication apart from these antibiotics were applied, it was concluded that fertility problems were due to the presence of these two Mycoplasma species. El objetivo de esta comunicación es describir un caso de subfertilidad y otras anomalías asociadas a la presencia de Mycoplasma spumans y Mycoplasma maculosum en un criadero de perros boyero de Berna. Después de la entrada de 2 perros del exterior, comenzaron a observarse algunos trastornos de la fertilidad, como apareamientos infructuosos, pérdidas de preñez y resultados anormales en el análisis espermático. Se realizaron 2 muestreos consecutivos (hisopados vaginales) de 3 y 2 perras con problemas, respectivamente, y se identificó M. spumans y M. maculosum mediante PCR y secuenciación. Después del tratamiento con doxiciclina y azitromicina durante 15 y 9 días, respectivamente, se lograron obtener preñeces exitosas y se revirtieron los resultados del espermograma. Considerando que no se detectaron otros agentes infecciosos causantes de problemas de subfertilidad y que no se aplicó ninguna medida de manejo ni otra medicación, aparte de los tratamientos antibióticos mencionados, se concluyó que los problemas de fertilidad se debieron a la presencia de estas 2 especies de Mycoplasma.