Regulation of serum microRNA expression by acupuncture in patients with diarrhea-predominant irritable bowel syndrome
Objective: To screen for differentially expressed serum microRNAs (miRNAs) in patients with diarrhea-predominant irritable bowel syndrome (IBS-D) compared with healthy participants and explore the mechanism of acupuncture in the treatment of IBS-D based on miRNAs. Methods: IBS-D patients that met th...
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Veröffentlicht in: | Acupuncture in medicine : journal of the British Medical Acupuncture Society 2022-02, Vol.40 (1), p.34-42 |
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Format: | Artikel |
Sprache: | eng |
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Zusammenfassung: | Objective:
To screen for differentially expressed serum microRNAs (miRNAs) in patients with diarrhea-predominant irritable bowel syndrome (IBS-D) compared with healthy participants and explore the mechanism of acupuncture in the treatment of IBS-D based on miRNAs.
Methods:
IBS-D patients that met the Rome III diagnostic criteria and age- and sex-matched healthy participants were enrolled between April 2017 and December 2017. Serum miRNA levels were initially determined using a TaqMan low-density array (TLDA) in pooled samples. Markedly altered miRNAs in IBS-D patients were subsequently validated using quantitative real-time polymerase chain reaction (qRT-PCR) on individual samples. All IBS-D patients accepted the acupuncture therapy for 6 weeks. The disease severity was assessed using the IBS symptom severity scale (IBS-SSS) questionnaire before and after treatment. After acupuncture, the patients’ serum was re-analyzed for altered expression of the miRNAs by qRT-PCR.
Results:
TLDA and qRT-PCR analysis revealed six upregulated miRNAs (miR-1305, miR-575, miR-149-5p, miR-190a-5p, miR-135a-5p, and miR-148a-3p; P |
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ISSN: | 0964-5284 1759-9873 |
DOI: | 10.1177/09645284211027892 |