Perforations of tri‐layer nitrile‐latex and natural rubber latex gloves during ex‐vivo equine intestinal anastomoses

Objective To compare perforation rates between tri‐layer nitrile‐latex and natural rubber latex surgical gloves after single‐layer end‐to‐end jejunojejunal anastomoses in equine cadavers. Study Design Prospective randomized ex‐vivo study. Sample Population Pairs of surgical gloves (n = 46) worn during jejunojejunal anastomoses. Methods Tri‐layer nitrile‐latex and rubber latex pairs of gloves were equally but randomly allocated to a right‐handed surgeon performing 46 single‐layer end‐to‐end jejunojejunal anastomoses on cadaveric material. Number and location of perforations were determined with the water leak test after each procedure. Ten unused pairs of both glove types were tested as controls. Results At least one perforation occurred in 41% (19/46) of the pairs of gloves. Glove perforations were present in 22% (5/23; 95% CI: 9–42) of tri‐layer glove pairs and 61% (14/23; 95% CI: 41–78) of the rubber glove pairs. The odds of glove perforation were 5.6 times (p = .009, 95% CI: 1.5–20.5) lower when tri‐layer rather than rubber gloves were used. The duration of procedure did not affect the risk of glove perforation (p = .679). No perforations were observed in the unused gloves. Conclusion Perforations were less common when the surgeon wore tri‐layer nitrile‐latex gloves rather than to the natural rubber latex gloves tested in this study. Clinical Relevance Tri‐layer nitrile‐latex gloves were more resistant to perforations in experimental settings; further studies may confirm that they are also superior in a clinical setting.