Genetic mapping reveals BjRf as a candidate gene controlling fertility restoration of the oxa CMS in Brassica juncea

Key message A candidate gene for male fertility restoration in Brassica juncea , BjRf , was isolated from a 23-kb interval on chromosome A05 using map-based cloning and BSA methods. The cytoplasmic male sterility/fertility restoration (CMS/ Rf ) system has been extensively used for heterosis in plants. It also provides valuable resources for studying mitochondrial–nuclear coevolution and interaction. The oxa CMS, which is a new CMS type reported in Brassica juncea ( B. juncea ), has been broadly used in the exploitation and application of heterosis in this species. However, the oxa CMS fertility restorer gene BjRf has not been reported. In this study, a stable restorer line was successfully constructed via continuous testcross and artificial selection. Besides, a new Rf gene was mapped in a 23-kb region on chromosome A05 in B. juncea with a genetic distance of 0.5 cM by the method incorporating bulk segregant analysis (BSA) and conventional map-based cloning. Finally, BjuA017917 , a non-PPR Rf gene encoding a guanosine nucleotide diphosphate dissociation inhibitor (GDI), is proposed to be the candidate gene for fertility restoration of the oxa CMS line in B. juncea . Moreover, a functional marker, CRY3, was developed for marker-assisted selection for Brassica juncea breeding.