Arabidopsis non‐host resistance PSS30 gene enhances broad‐spectrum disease resistance in the soybean cultivar Williams 82

Summary Non‐host resistance (NHR), which protects all members of a plant species from non‐adapted or non‐host plant pathogens, is the most common form of plant immunity. NHR provides the most durable and robust form of broad‐spectrum immunity against non‐adaptive pathogens pathogenic to other crop species. In a mutant screen for loss of Arabidopsis (Arabidopsis thaliana) NHR against the soybean (Glycine max (L.) Merr.) pathogen Phytophthora sojae, the Phytophthora sojae‐susceptible 30 (pss30) mutant was identified. The pss30 mutant is also susceptible to the soybean pathogen Fusarium virguliforme. PSS30 encodes a folate transporter, AtFOLT1, which was previously localized to chloroplasts and implicated in the transport of folate from the cytosol to plastids. We show that two Arabidopsis folate biosynthesis mutants with reduced folate levels exhibit a loss of non‐host immunity against P. sojae. As compared to the wild‐type Col‐0 ecotype, the steady‐state folate levels are reduced in the pss1, atfolt1 and two folate biosynthesis mutants, suggesting that folate is required for non‐host immunity. Overexpression of AtFOLT1 enhances immunity of transgenic soybean lines against two serious soybean pathogens, the fungal pathogen F. virguliforme and the soybean cyst nematode (SCN) Heterodera glycines. Transgenic lines showing enhanced SCN resistance also showed increased levels of folate accumulation. This study thus suggests that folate contributes to non‐host plant immunity and that overexpression of a non‐host resistance gene could be a suitable strategy for generating broad‐spectrum disease resistance in crop plants. Significance Statement Plant diseases are chronic deterrents of crop production, causing crop losses of billions of dollars annually. Here we identified the Arabidopsis non‐host resistance gene PSS30, which encodes a folate transporter. Expression of PSS30 in soybean (Glycine max) generates broad‐spectrum disease resistance against its two most serious pathogens, Fusarium virguliforme and Heterodera glycines, which together cause annual soybean yield losses valued at close to 2 billion dollars.