Glycol Chitosan Functionalized with a Gd(III) Chelate as a Redox‐responsive Magnetic Resonance Imaging Probe to Label Cell Embedding Alginate Capsules

One possibility for the non‐invasive imaging of encapsulated cell grafts is to label the lumen of cell embedding capsules with a redox‐responsive probe, as an increased extracellular reducing potential can be considered as a marker of hypoxia‐induced necrosis. A Gd(III)‐HPDO3A‐like chelate has been...

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Veröffentlicht in:Chemistry : a European journal 2021-08, Vol.27 (48), p.12289-12293
Hauptverfasser: Padovan, Sergio, Carrera, Carla, Catanzaro, Valeria, Grange, Cristina, Koni, Malvina, Digilio, Giuseppe
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Sprache:eng
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Zusammenfassung:One possibility for the non‐invasive imaging of encapsulated cell grafts is to label the lumen of cell embedding capsules with a redox‐responsive probe, as an increased extracellular reducing potential can be considered as a marker of hypoxia‐induced necrosis. A Gd(III)‐HPDO3A‐like chelate has been conjugated to glycol‐chitosan through a redox‐responsive disulphide bond to obtain a contrast agent for Magnetic Resonance Imaging (MRI). Such a compound can be interspersed with fibroblasts within the lumen of alginate‐chitosan capsules. Increasing reducing conditions within the extracellular microenvironment lead to the reductive cleavage of the disulphide bond and to the release of gadolinium in the form of a low molecular weight, non‐ionic chelate. The efflux of such chelate from capsules is readily detected by a decrease of contrast enhancement in T1‐weighted MR images. A Gd(III)‐HPDO3A‐like chelate has been conjugated to glycol‐chitosan through a disulphide bond to obtain a redox‐responsive contrast agent for Magnetic Resonance Imaging (MRI), that can be used to label the lumen of alginate‐based cell embedding capsules. The increase of reducing potential within the capsule microenvironment, that is related to hypoxia, can be readily detected by a decrease of MRI contrast enhancement.
ISSN:0947-6539
1521-3765
DOI:10.1002/chem.202101657