Simultaneous enhancement of treatment performance and energy recovery using pyrite as anodic filling material in constructed wetland coupled with microbial fuel cells

•Pyrite is used as an inexpensive anodic filling material (PyAno) for CW-MFCs.•PyAno can facilitate COD and NH4+-N removal of CW-MFCs.•PyAno can increase the electricity production of CW-MFCs.•Conductivity of pyrite can enlarge the scope of anode-influence.•The iron redox cycling is shaped by the coaction of pyrite oxidation and DIR. Constructed wetland coupled with microbial fuel cells (CW-MFCs) are a promising technology for sustainable wastewater treatment. However, the performance of CW-MFCs has long been constrained by the limited size of its anode. In this study, we developed an alternative CW-MFC configuration that uses inexpensive natural conductive pyrite as an anodic filling material (PyAno) to extend the electroactive scope of the anode. As a result, the PyAno configuration significantly facilitated the removal of chemical oxygen demand, ammonium nitrogen, total nitrogen, and total phosphorus. Meanwhile, the PyAno increased the maximum power density by 52.7% as compared to that of the quartz sand control. Further, a typical exoelectrogen Geobacter was found enriched in the anodic zone of PyAno, indicating that the electroactive scope was extended by conductive pyrite. In addition, a substantial electron donating potential was observed for the anodic filling material of PyAno, which explained the higher electricity output. Meanwhile, a higher dissimilatory iron reducing potential was observed for the anodic sediment of PyAno, demonstrating the integrity of an iron redox cycling in the system and its promotive effect for the wastewater treatment. Together, these results implied that the PyAno CW-MFCs can be a competitive technology to enhance wastewater treatment and energy recovery simultaneously. [Display omitted]