Pseudoxanthomonas beigongshangi sp. nov., a novel bacteria with predicted nitrite and nitrate reduce ability isolated from pit mud of Baijiu

A Gram-negative and rod-shape bacterium designated REN9 T , was isolated from pit mud of Baijiu in Sichuan, China. The 16S rRNA sequence of strain REN9 T had a high similarity to Pseudoxanthomonas indica P15 T (99.21%), P. mexicana AMX 26B T (97.74%) and P. japonensis 12-3 T (97.43%). Phylogenetic analysis showed that REN9 T belongs to the genus Pseudoxanthomonas and formed distinct cluster. The ANI and DDH values between strains REN9 T and P15 T were 80.94% and 24%, respectively. Strain REN9 T grew optimally at 37 °C, pH 7.0 and 2% NaCl. PE (phosphatidylethanolamine), PG (phosphatidylglycerol) and DPG (diphosphatidylglycerol) were the major polar lipids of REN9 T . Ubiquinone 8 (Q-8) was the predominant quinone, and Iso-C 15:0 (64.32%), anteiso-C 15:0 (12.04%) and Iso-C 14:0 (4.56%) were the majority fatty acids. The DNA G + C content of strain REN9 T was 67.3 mol%. Genomic analysis showed that strain REN9 T had two secondary metabolite biosynthesis gene clusters. Moreover, strain REN9 T had 43 glycoside hydrolases, 41 glycosyl transferases and 41 carbohydrate esterases. Based on the polyphasic taxonomic analysis, strain REN9 T is recommended as a novel species within the genus Pseudoxanthomonas , for which the name Pseudoxanthomonas beigongshangi is proposed. The type stain is REN9 T (= JCM 33961 T = GDMCC 1.2210 T ).