Analysis of expression characteristics of soybean leaf and root tissue-specific promoters in Arabidopsis and soybean
The characterization of tissue-specific promoters is critical for studying the functions of genes in a given tissue/organ. To study tissue-specific promoters in soybean, we screened tissue-specific expressed genes using published soybean RNA-Seq-based transcriptome data coupled with RT-PCR analysis....
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Veröffentlicht in: | Transgenic research 2021-12, Vol.30 (6), p.799-810 |
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Sprache: | eng |
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Zusammenfassung: | The characterization of tissue-specific promoters is critical for studying the functions of genes in a given tissue/organ. To study tissue-specific promoters in soybean, we screened tissue-specific expressed genes using published soybean RNA-Seq-based transcriptome data coupled with RT-PCR analysis. We cloned the promoters of three genes,
GmADR1
,
GmBTP1
, and
GmGER1
, and constructed their corresponding β-Glucuronidase (
GUS
) promoter-GUS reporter vectors. We generated transgenic
Arabidopsis
plants and examined the expression patterns of these promoters by GUS staining and RT-PCR analysis. We also transformed the promoter-
GUS
reporter vectors into soybean to obtain hairy roots, and examined promoter expression by GUS staining. We found a root-specific expression pattern of
GmADR1
and
GmBTP1
in both
Arabidopsis
and soybean, and the promoter of
GmGER1
showed a leaf-specific pattern in transgenic
Arabidopsis
plants. To test the potential utility of these promoters in soybean improvement by transgenic means, we used the
GmADR1
promoter to drive expression of a salt resistance gene in soybean,
GmCaM4
, by generating transgenic soybean plants. We found that the transgenic plants had significantly enhanced salt tolerance compared to non-transformed wild-type, suggesting that introducing endogenous promoters by transgenic means can drive the expression of functional genes in specific tissues and organs in soybean. |
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ISSN: | 0962-8819 1573-9368 |
DOI: | 10.1007/s11248-021-00266-7 |