Vimentin rearrangement by phosphorylation is beneficial for porcine reproductive and respiratory syndrome virus replication in vitro

•PRRSV infection induces vimentin rearrangement during post-entry stage.•Vimentin enwraps PRRSV replication complex at the perinuclear site.•Vimentin rearrangement is beneficial for PRRSV replication.•Vimentin (Ser38) phosphorylation by CaMKIIγ is responsible for rearrangement. Vimentin, a member of...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Veterinary microbiology 2021-08, Vol.259, p.109133, Article 109133
Hauptverfasser: Zheng, Xin-xian, Li, Rui, Qiao, Songlin, Chen, Xin-xin, Zhang, Longxiang, Lu, Qingxia, Xing, Guangxu, Zhou, En-min, Zhang, Gaiping
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:•PRRSV infection induces vimentin rearrangement during post-entry stage.•Vimentin enwraps PRRSV replication complex at the perinuclear site.•Vimentin rearrangement is beneficial for PRRSV replication.•Vimentin (Ser38) phosphorylation by CaMKIIγ is responsible for rearrangement. Vimentin, a member of intermediate filaments, has been documented to be involved in viral infections. Despite several studies focusing on its involvement in porcine reproductive and respiratory syndrome virus (PRRSV) infection, the detailed mechanisms by which vimentin takes effect remain to be fully elucidated. In the present study, we identified a previously unrecognized role of vimentin rearrangement in PRRSV replication. We monitored that PRRSV infection induced vimentin reorganization during post-entry stage, which was beneficial for viral replication. In detail, the serine residue of vimentin was phosphorylated at position 38 (Ser38) by calcium calmodulin-dependent protein kinase II gamma (CaMKIIγ), and vimentin filaments reorganized into cage-like structures enwrapping PRRSV replication complex (RC) at the perinuclear location. Taken together, these results expand the knowledge of PRRSV replication, and provide novel targets for prevention and control of PRRSV.
ISSN:0378-1135
1873-2542
1873-2542
DOI:10.1016/j.vetmic.2021.109133