Evaluation of the MICRONAUT MIC-strip colistin assay for colistin susceptibility testing of carbapenem-resistant Acinetobacter baumannii and Enterobacterales

•The MICRONAUT MIC-Strip Colistin enables high performance antimicrobial susceptibility testing against colistin by using the broth microdilution method for carbapenem-resistant Enterobacterales and Acinetobacter baumannii.•MICRONAUT MIC-Strip Colistin had 98.5% sensitivity and 99.5% specificity. Isolates with MICs close to the colistin clinical breakpoint exhibited complete agreement.•Standardized MIC determination of colistin in routine laboratory diagnostics according to EUCAST-CLSI recommendations.•The strip format allows for an economic use of the AST adapted to the individual laboratory diagnostic requirements. We sought to evaluate the MICRONAUT MIC-Strip colistin (MMS) assay, a commercial broth microdilution (BMD) panel, using 273 carbapenem-resistant Acinetobacter baumannii and Enterobacterales isolates. BMD was used as gold standard. MMS had 98.5% sensitivity and 99.5% specificity. All 37 isolates with MICs close to the breakpoint exhibited complete agreement.