Visualization of Spatial Distribution of Spermatogenesis in Mouse Testes Using Creatine Chemical Exchange Saturation Transfer Imaging
Background When determining treatment strategies for male infertility, it is important to evaluate spermatogenesis and its spatial distribution in the testes. Purpose To investigate the usefulness of creatine chemical exchange saturation transfer (CrCEST) imaging for evaluating spermatogenesis and i...
Gespeichert in:
Veröffentlicht in: | Journal of magnetic resonance imaging 2021-11, Vol.54 (5), p.1457-1465 |
---|---|
Hauptverfasser: | , , , , , , , , |
Format: | Artikel |
Sprache: | eng |
Schlagworte: | |
Online-Zugang: | Volltext |
Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
Zusammenfassung: | Background
When determining treatment strategies for male infertility, it is important to evaluate spermatogenesis and its spatial distribution in the testes.
Purpose
To investigate the usefulness of creatine chemical exchange saturation transfer (CrCEST) imaging for evaluating spermatogenesis and its spatial distribution.
Study Type
Prospective.
Animal Model
C57BL/6 control mice (n = 5) and model mice of male infertility induced by whole testis X‐ray irradiation (n = 11) or localized X‐ray irradiation to lower regions of testes (n = 3).
Field Strength/Sequence
A 11.7‐T vertical‐bore magnetic resonance imaging (MRI)/segmented fast low‐angle shot acquisition for CEST.
Assessment
The magnetization transfer ratio for the CrCEST effect (MTRCr*) was calculated in each testis of the control mice and X‐ray irradiation model mice at 10, 15, 20, and 30 days after irradiation. Correlation analysis was performed between MTRCr* and Johnsen's score, a histological score for spermatogenesis. In the localized X‐ray irradiation model, regional MTRCr* and Johnsen's score were calculated for correlation analysis.
Statistical Tests
Unpaired t‐test, one‐way analysis of variance with Tukey's HSD test and Pearson's correlation analysis. A P value |
---|---|
ISSN: | 1053-1807 1522-2586 |
DOI: | 10.1002/jmri.27734 |