Interaction between CD9 and PI3K‑p85 activates the PI3K/AKT signaling pathway in B‑lineage acute lymphoblastic leukemia

Our previous study has shown that CD9 knockdown could suppress cell proliferation, adhesion, migration and invasion, and promote apoptosis and the cytotoxicity of chemotherapeutic drugs in the B-lineage acute lymphoblastic leukemia (B-ALL) cell line SUP-B15. In this study, we further investigated the molecular mechanism underlying the effects of CD9 on leukemic cell progression and the efficacy of chemotherapeutic agents in B-ALL cells. Using the CD9-knockdown SUP-B15 cells, we demonstrated that the silencing of the CD9 gene significantly reduced the expression of phosphorylated-phosphatidylinositol-3 kinase (p-PI3K), phosphorylated-protein kinase B (p-AKT), P-glycoprotein (P-gp), multidrug resistance-associated protein 1 (MRP1), breast cancer resistance protein (BCRP), matrix met alloproteinase 2 (MMP2) and phosphorylated-focal adhesion kinase (p-EAK). In addition, glutathione S-transferase (GST) pull-down assay showed the binding between CD9 and both PI3K-p85[alpha] and pI3K-[rho]85[beta] in vitro, while co-immunoprecipitation assay showed the binding between CD9 and both PI3K-p85[alpha] and pI3K-[rho]85[beta] in vivo. Furthermore, the PI3K/AKT inhibitor LY294002 mirrored the effects of CD9 knockdown in SUP-B15 cells. Taken together, these findings demonstrated that CD9 activates the PI3K/AKT signaling pathway through direct interaction with PI3K-p85 in B-ALL cells. Our data provide evidence for the inhibition of the PI3K/AKT pathway as a novel therapeutic option in CD9 antigen-positive B-ALL.