Two newly identified Haematococcus strains efficiently accumulated radioactive cesium over higher astaxanthin production

In this study, we investigated the morphological, genomic and bioaccumulation characteristics of two isolated Haematococcus strains (namely Goyang and Sogang), which were newly discovered in South Korea. Morphological analysis revealed that the isolated strains were unicellular and bi-flagellated green microalgae that formed thickened walls at the palmelloid or red-cyst phase. Phylogenetic analysis of 18S rRNA and rbcL gDNA sequences demonstrated that both strains were taxonomically related to the genus Haematococcus. The two strains showed growth pattern that was similar to a typical Haematococcus strain, and accumulated astaxanthin within 48 h of exposure to intensive light. Both red-cyst cells effectively removed radioactive cesium to more than 50% within 48 h from low-level cesium-contaminated water of 5 Bq/ml concentration. The cesium-accumulation mechanism is largely associated with the replacement of cellular potassium in thick cell walls during biouptake, and the cesium-removal rate highly depends on the corresponding astaxanthin accumulation involving the potassium-transporting protein (P-type ATPase). •Isolation and Identification of two new Haematococcus strains discovered in South Korea.•Phylogenetic analysis demonstrated that both strains were taxonomically related to the genus Haematococcus.•High cesium-removal by the replacement of cellular potassium in 5 Bq/mL radioactive water within 48 h.•The original finding of cesium-biouptake rate depending highly on astaxanthin accumulation.