Massilia horti sp. nov. and Noviherbaspirillum arenae sp. nov., two novel soil bacteria of the Oxalobacteraceae

We isolated two new soil bacteria: ONC3 T (from garden soil in NC, USA; LMG 31738 T =NRRL B-65553 T ) and M1 T (from farmed soil in MI, USA; NRRL B-65551 T =ATCC TSD-197 T =LMG 31739 T ) and characterized their metabolic phenotype based on Biolog, MALDI-TOF MS and fatty acid analyses, and compared 16S rRNA and whole genome sequences to other members of the Oxalobacteraceae after sequencing on an Illumina Nextera platform. Based on the results of 16S rRNA sequence analysis, ONC3 T shows the highest sequence similarity to Massilia solisilvae J18 T (97.8 %), Massilia terrae J11 T (97.7 %) and Massilia agilis J9 T (97.3 %). Strain M1 T is most closely related to Noviherbaspirillum denitrificans TSA40 T , Noviherbaspirillum agri K-1-15 T and Noviherbaspirillum autotrophicum TSA66 T (sequence identity of 98.2, 98.0 and 97.8 %, respectively). The whole genome of ONC3 T has an assembled size of 5.62 Mbp, a G+C content of 63.8 mol% and contains 5104 protein-coding sequences, 56 tRNA genes and two rRNA operons. The genome of M1 T has a length of 4.71 MBp, a G+C content of 63.81 mol% and includes 4967 protein-coding genes, two rRNA operons and 44 tRNA genes. Whole genome comparisons identified Massilia sp. WG5 with a 79.3 % average nucleotide identity (ANI) and 22.6 % digital DNA–DNA hybridization (dDDH), and Massilia sp. UBA11196 with 78.2 % average amino acid identity (AAI) as the most closely related species to ONC3 T . M1 T is most closely related to N. autotrophicum TSA66 T with an ANI of 80.27 %, or N. denitrificans TSA40 T with a dDDH of 22.3 %. The application of community-accepted standards such as