Structure elucidation and gene cluster annotation of the O-antigen of Pseudomonas veronii SHC-8-1 containing 2-acetamido-2,4,6-trideoxy-4-(3,5-dihydroxyhexanoylamino)-d-glucose

O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: [Display omitted] where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-tri...

Ausführliche Beschreibung

Gespeichert in:
Bibliographische Detailangaben
Veröffentlicht in:Carbohydrate research 2021-06, Vol.504, p.108306, Article 108306
Hauptverfasser: Perepelov, Andrei V., Filatov, Andrei V., Shashkov, Alexander S., Grouzdev, Denis S., Babich, Tamara L., Popova, Nadezhda M., Safonov, Alexey V.
Format: Artikel
Sprache:eng
Schlagworte:
Online-Zugang:Volltext
Tags: Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
Beschreibung
Zusammenfassung:O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: [Display omitted] where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-trideoxy-dglucose (Bacillosamine) in which N-2 is acetylated and N-4 is acylated with 3,5-dihydroxyhexanoic acid (dHh). The O-antigen gene cluster of Pseudomonas veronii SHC-8-1 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure. [Display omitted] •The strain P. veronii SHC-8-1 was isolated nearby radioactive waste repository.•P. veronii SHC-8-1 can play an important role in immobilization of radionuclides.•The O-specific polysaccharide structure of P. veronii SHC-8-1 was established.•The OPS was found to contain rarely occurring amino sugar derivative.•The O-antigen gene cluster of P. veronii SHC-8-1 was sequenced and annotated.
ISSN:0008-6215
1873-426X
1873-426X
DOI:10.1016/j.carres.2021.108306