Structure elucidation and gene cluster annotation of the O-antigen of Pseudomonas veronii SHC-8-1 containing 2-acetamido-2,4,6-trideoxy-4-(3,5-dihydroxyhexanoylamino)-d-glucose

O-polysaccharide (O-antigen, OPS) was isolated from the lipopolysaccharide of Pseudomonas veronii SHC-8-1 and studied by component analyses and 1D and 2D NMR spectroscopy. The following structure of the O-polysaccharide was established: [Display omitted] where QuipNAc4N(dHh) is 2,4-diamino-2,4,6-trideoxy-dglucose (Bacillosamine) in which N-2 is acetylated and N-4 is acylated with 3,5-dihydroxyhexanoic acid (dHh). The O-antigen gene cluster of Pseudomonas veronii SHC-8-1 has been sequenced. The gene functions were tentatively assigned by comparison with sequences in the available databases and found to be in agreement with the OPS structure. [Display omitted] •The strain P. veronii SHC-8-1 was isolated nearby radioactive waste repository.•P. veronii SHC-8-1 can play an important role in immobilization of radionuclides.•The O-specific polysaccharide structure of P. veronii SHC-8-1 was established.•The OPS was found to contain rarely occurring amino sugar derivative.•The O-antigen gene cluster of P. veronii SHC-8-1 was sequenced and annotated.