Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey ( Nasalis larvatus )
Three strains (YZ01 , YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey ( ) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged...
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| Veröffentlicht in: | International journal of systematic and evolutionary microbiology 2021-04, Vol.71 (4) |
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| container_title | International journal of systematic and evolutionary microbiology |
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| creator | Suzuki-Hashido, Nami Tsuchida, Sayaka Hayakawa, Takashi Sakamoto, Mitsuo Azumano, Akinori Seino, Satoru Matsuda, Ikki Ohkuma, Moriya Ushida, Kazunari |
| description | Three strains (YZ01
, YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey (
) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genus
. Based on the sequence similarity of the 16S rRNA gene,
subsp.
JCM 15610
was the closest phylogenetic neighbour to YZ01
. Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit (
) and phenylalanyl-tRNA synthase alpha subunit (
) also indicated that the novel strains belonged to the genus
. The average nucleotide identity and digital DNA-DNA hybridization (dDDH) between
subsp.
and YZ01
were 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01
, YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genus
, indicating that it belonged to a new species. The DNA G+C content of strain YZ01
was 51.6 mol%. The major fatty acids were C
and C
ω9
. Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01
, YZ02 and YZ03 represent a novel species of the genus
, for which the name
sp. nov. is proposed with the type strain YZ01
(=JCM 33769
=DSM 110539
). |
| doi_str_mv | 10.1099/ijsem.0.004787 |
| format | Article |
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, YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey (
) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genus
. Based on the sequence similarity of the 16S rRNA gene,
subsp.
JCM 15610
was the closest phylogenetic neighbour to YZ01
. Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit (
) and phenylalanyl-tRNA synthase alpha subunit (
) also indicated that the novel strains belonged to the genus
. The average nucleotide identity and digital DNA-DNA hybridization (dDDH) between
subsp.
and YZ01
were 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01
, YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genus
, indicating that it belonged to a new species. The DNA G+C content of strain YZ01
was 51.6 mol%. The major fatty acids were C
and C
ω9
. Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01
, YZ02 and YZ03 represent a novel species of the genus
, for which the name
sp. nov. is proposed with the type strain YZ01
(=JCM 33769
=DSM 110539
).</description><identifier>ISSN: 1466-5026</identifier><identifier>EISSN: 1466-5034</identifier><identifier>DOI: 10.1099/ijsem.0.004787</identifier><identifier>PMID: 33906706</identifier><language>eng</language><publisher>England</publisher><subject>Animals ; Animals, Zoo - microbiology ; Bacterial Typing Techniques ; Base Composition ; DNA, Bacterial - genetics ; DNA-Directed RNA Polymerases - genetics ; Fatty Acids - chemistry ; Genes, Bacterial ; Japan ; Lactobacillus - classification ; Lactobacillus - isolation & purification ; Nucleic Acid Hybridization ; Phylogeny ; Presbytini - microbiology ; RNA, Ribosomal, 16S - genetics ; Sequence Analysis, DNA ; Stomach - microbiology</subject><ispartof>International journal of systematic and evolutionary microbiology, 2021-04, Vol.71 (4)</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c361t-33de3377e176b8466bfa27e550f9ed5edaf961fe427d33dc23646046aea5aa9d3</citedby><cites>FETCH-LOGICAL-c361t-33de3377e176b8466bfa27e550f9ed5edaf961fe427d33dc23646046aea5aa9d3</cites><orcidid>0000-0002-9934-7324 ; 0000-0002-3622-4642 ; 0000-0002-0861-7801 ; 0000-0002-3155-4538 ; 0000-0003-3753-990X ; 0000-0002-1037-0350</orcidid></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,3744,27922,27923</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/33906706$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Suzuki-Hashido, Nami</creatorcontrib><creatorcontrib>Tsuchida, Sayaka</creatorcontrib><creatorcontrib>Hayakawa, Takashi</creatorcontrib><creatorcontrib>Sakamoto, Mitsuo</creatorcontrib><creatorcontrib>Azumano, Akinori</creatorcontrib><creatorcontrib>Seino, Satoru</creatorcontrib><creatorcontrib>Matsuda, Ikki</creatorcontrib><creatorcontrib>Ohkuma, Moriya</creatorcontrib><creatorcontrib>Ushida, Kazunari</creatorcontrib><title>Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey ( Nasalis larvatus )</title><title>International journal of systematic and evolutionary microbiology</title><addtitle>Int J Syst Evol Microbiol</addtitle><description>Three strains (YZ01
, YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey (
) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genus
. Based on the sequence similarity of the 16S rRNA gene,
subsp.
JCM 15610
was the closest phylogenetic neighbour to YZ01
. Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit (
) and phenylalanyl-tRNA synthase alpha subunit (
) also indicated that the novel strains belonged to the genus
. The average nucleotide identity and digital DNA-DNA hybridization (dDDH) between
subsp.
and YZ01
were 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01
, YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genus
, indicating that it belonged to a new species. The DNA G+C content of strain YZ01
was 51.6 mol%. The major fatty acids were C
and C
ω9
. Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01
, YZ02 and YZ03 represent a novel species of the genus
, for which the name
sp. nov. is proposed with the type strain YZ01
(=JCM 33769
=DSM 110539
).</description><subject>Animals</subject><subject>Animals, Zoo - microbiology</subject><subject>Bacterial Typing Techniques</subject><subject>Base Composition</subject><subject>DNA, Bacterial - genetics</subject><subject>DNA-Directed RNA Polymerases - genetics</subject><subject>Fatty Acids - chemistry</subject><subject>Genes, Bacterial</subject><subject>Japan</subject><subject>Lactobacillus - classification</subject><subject>Lactobacillus - isolation & purification</subject><subject>Nucleic Acid Hybridization</subject><subject>Phylogeny</subject><subject>Presbytini - microbiology</subject><subject>RNA, Ribosomal, 16S - genetics</subject><subject>Sequence Analysis, DNA</subject><subject>Stomach - microbiology</subject><issn>1466-5026</issn><issn>1466-5034</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2021</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9UMtOwzAQtBCIlsKVI_KxSCTYceI0R1Txkiq4wDnaOGs1xYmDnVTq32PawmlXq5nZmSHkmrOYs6K4bzYe25jFjKX5Ij8hU55KGWVMpKf_eyIn5ML7DWPhwNg5mQhRMJkzOSVuBWqwFajGmNHTDjyYpm489X1MO7uN72jjrYEBa6qdbemwRqqtQz_YFtSaWk2BKuiHZou0d7ayXgV6a7sv3NE5fdsremrAbWEIL24vyZkG4_HqOGfk8-nxY_kSrd6fX5cPq0gJyYdIiBqFyHPkuawWIUmlIckxy5gusM6wBl1IrjFN8jpgVSJkSJdKQMgAilrMyPygG1x9j8Fw2TZeoTHQoR19mWS8EFzKNA3Q-ABVznrvUJe9a1pwu5Kz8rfnct9zycpDz4Fwc9Qeqxbrf_hfseIH5R161w</recordid><startdate>20210427</startdate><enddate>20210427</enddate><creator>Suzuki-Hashido, Nami</creator><creator>Tsuchida, Sayaka</creator><creator>Hayakawa, Takashi</creator><creator>Sakamoto, Mitsuo</creator><creator>Azumano, Akinori</creator><creator>Seino, Satoru</creator><creator>Matsuda, Ikki</creator><creator>Ohkuma, Moriya</creator><creator>Ushida, Kazunari</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><orcidid>https://orcid.org/0000-0002-9934-7324</orcidid><orcidid>https://orcid.org/0000-0002-3622-4642</orcidid><orcidid>https://orcid.org/0000-0002-0861-7801</orcidid><orcidid>https://orcid.org/0000-0002-3155-4538</orcidid><orcidid>https://orcid.org/0000-0003-3753-990X</orcidid><orcidid>https://orcid.org/0000-0002-1037-0350</orcidid></search><sort><creationdate>20210427</creationdate><title>Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey ( Nasalis larvatus )</title><author>Suzuki-Hashido, Nami ; Tsuchida, Sayaka ; Hayakawa, Takashi ; Sakamoto, Mitsuo ; Azumano, Akinori ; Seino, Satoru ; Matsuda, Ikki ; Ohkuma, Moriya ; Ushida, Kazunari</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c361t-33de3377e176b8466bfa27e550f9ed5edaf961fe427d33dc23646046aea5aa9d3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2021</creationdate><topic>Animals</topic><topic>Animals, Zoo - microbiology</topic><topic>Bacterial Typing Techniques</topic><topic>Base Composition</topic><topic>DNA, Bacterial - genetics</topic><topic>DNA-Directed RNA Polymerases - genetics</topic><topic>Fatty Acids - chemistry</topic><topic>Genes, Bacterial</topic><topic>Japan</topic><topic>Lactobacillus - classification</topic><topic>Lactobacillus - isolation & purification</topic><topic>Nucleic Acid Hybridization</topic><topic>Phylogeny</topic><topic>Presbytini - microbiology</topic><topic>RNA, Ribosomal, 16S - genetics</topic><topic>Sequence Analysis, DNA</topic><topic>Stomach - microbiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Suzuki-Hashido, Nami</creatorcontrib><creatorcontrib>Tsuchida, Sayaka</creatorcontrib><creatorcontrib>Hayakawa, Takashi</creatorcontrib><creatorcontrib>Sakamoto, Mitsuo</creatorcontrib><creatorcontrib>Azumano, Akinori</creatorcontrib><creatorcontrib>Seino, Satoru</creatorcontrib><creatorcontrib>Matsuda, Ikki</creatorcontrib><creatorcontrib>Ohkuma, Moriya</creatorcontrib><creatorcontrib>Ushida, Kazunari</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of systematic and evolutionary microbiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Suzuki-Hashido, Nami</au><au>Tsuchida, Sayaka</au><au>Hayakawa, Takashi</au><au>Sakamoto, Mitsuo</au><au>Azumano, Akinori</au><au>Seino, Satoru</au><au>Matsuda, Ikki</au><au>Ohkuma, Moriya</au><au>Ushida, Kazunari</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey ( Nasalis larvatus )</atitle><jtitle>International journal of systematic and evolutionary microbiology</jtitle><addtitle>Int J Syst Evol Microbiol</addtitle><date>2021-04-27</date><risdate>2021</risdate><volume>71</volume><issue>4</issue><issn>1466-5026</issn><eissn>1466-5034</eissn><abstract>Three strains (YZ01
, YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey (
) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genus
. Based on the sequence similarity of the 16S rRNA gene,
subsp.
JCM 15610
was the closest phylogenetic neighbour to YZ01
. Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit (
) and phenylalanyl-tRNA synthase alpha subunit (
) also indicated that the novel strains belonged to the genus
. The average nucleotide identity and digital DNA-DNA hybridization (dDDH) between
subsp.
and YZ01
were 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01
, YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genus
, indicating that it belonged to a new species. The DNA G+C content of strain YZ01
was 51.6 mol%. The major fatty acids were C
and C
ω9
. Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01
, YZ02 and YZ03 represent a novel species of the genus
, for which the name
sp. nov. is proposed with the type strain YZ01
(=JCM 33769
=DSM 110539
).</abstract><cop>England</cop><pmid>33906706</pmid><doi>10.1099/ijsem.0.004787</doi><orcidid>https://orcid.org/0000-0002-9934-7324</orcidid><orcidid>https://orcid.org/0000-0002-3622-4642</orcidid><orcidid>https://orcid.org/0000-0002-0861-7801</orcidid><orcidid>https://orcid.org/0000-0002-3155-4538</orcidid><orcidid>https://orcid.org/0000-0003-3753-990X</orcidid><orcidid>https://orcid.org/0000-0002-1037-0350</orcidid></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1466-5026 |
| ispartof | International journal of systematic and evolutionary microbiology, 2021-04, Vol.71 (4) |
| issn | 1466-5026 1466-5034 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_2519316644 |
| source | MEDLINE; Microbiology Society; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Animals Animals, Zoo - microbiology Bacterial Typing Techniques Base Composition DNA, Bacterial - genetics DNA-Directed RNA Polymerases - genetics Fatty Acids - chemistry Genes, Bacterial Japan Lactobacillus - classification Lactobacillus - isolation & purification Nucleic Acid Hybridization Phylogeny Presbytini - microbiology RNA, Ribosomal, 16S - genetics Sequence Analysis, DNA Stomach - microbiology |
| title | Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey ( Nasalis larvatus ) |
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