Lactobacillus nasalidis sp. nov., isolated from the forestomach of a captive proboscis monkey ( Nasalis larvatus )

Three strains (YZ01 , YZ02 and YZ03) of Gram-stain-positive, facultatively anaerobic rods were isolated from the forestomach contents collected from a captive male proboscis monkey ( ) at Yokohama Zoo in Japan. Phylogenetic analysis of the 16S rRNA gene sequences revealed that these strains belonged to the genus . Based on the sequence similarity of the 16S rRNA gene, subsp. JCM 15610 was the closest phylogenetic neighbour to YZ01 . Sequence analyses of two partial concatenated housekeeping genes, the RNA polymerase alpha subunit ( ) and phenylalanyl-tRNA synthase alpha subunit ( ) also indicated that the novel strains belonged to the genus . The average nucleotide identity and digital DNA-DNA hybridization (dDDH) between subsp. and YZ01 were 85.9 and 31.4 %, respectively. The phylogenetic tree based on the whole genomic data of strains YZ01 , YZ02 and YZ03 suggested that these three strains formed a single monophyletic cluster in the genus , indicating that it belonged to a new species. The DNA G+C content of strain YZ01 was 51.6 mol%. The major fatty acids were C and C ω9 . Therefore, based on phylogenetic, phenotypic and physiological evidence, strains YZ01 , YZ02 and YZ03 represent a novel species of the genus , for which the name sp. nov. is proposed with the type strain YZ01 (=JCM 33769 =DSM 110539 ).