GmST1, which encodes a sulfotransferase, confers resistance to soybean mosaic virus strains G2 and G3

Soybean mosaic virus (SMV) is one of the most widespread and devastating viral diseases worldwide. The genetic architecture of qualitative resistance to SMV in soybean remains unclear. Here, the Rsvg2 locus was identified as underlying soybean resistance to SMV by genome‐wide association and linkage analyses. Fine mapping results showed that soybean resistance to SMV strains G2 and G3 was controlled by a single dominant gene, GmST1, on chromosome 13, encoding a sulfotransferase (SOT). A key variation at position 506 in the coding region of GmST1 associated with the structure of the encoded SOT and changed SOT activity levels between RSVG2‐S and RSVG2‐R alleles. In RSVG2‐S allele carrier “Hefeng25”, the overexpression of GmST1 carrying the RSVG2‐R allele from the SMV‐resistant line “Dongnong93‐046” conferred resistance to SMV strains G2 and G3. Compared to Hefeng25, the accumulation of SMV was decreased in transgenic plants carrying the RSVG2‐R allele. SMV infection differentiated both the accumulation of jasmonates and expression patterns of genes involved in jasmonic acid (JA) signalling, biosynthesis and catabolism in RSVG2‐R and RSVG2‐S allele carriers. This characterization of GmST1 suggests a new scenario explaining soybean resistance to SMV. GmST1, encoding a sulfotransferase (SOT), was isolated from Rsvg2 locus underlying soybean resistance to mosaic virus (SMV). A key variation in the coding region of GmST1 changed the structure of the encoded SOT between the RSVG2‐S and RSVG2‐R alleles. The overexpression of GmST1 carrying the RSVG2‐R allele conferred resistance to SMV strains G2 and G3. GmST1 mediated SMV resistance was related to genes involved in JA signaling, biosynthesis and catabolism pathways.