Elizabethkingia argenteiflava sp. nov., isolated from the pod of soybean, Glycine max

Soybean pods, separated and enclosed from the outside environment, are considered a suitable place to find new microbes. A Gram-stain-negative, aerobic bacterium, bacterial strain (YB22 ) was isolated from the pod of (soybean) collected from a rural area in Republic of Korea and characterized by using polyphasic taxonomy. Cells of the strain were rod-shaped (approximately 0.4-0.6 µm wide and 4.0-5.0 µm long), non-flagellated and formed silver-yellow colonies. Cells grew at 25-35 °C (optimum, 28-30 °C), at pH 5.0-9.0 (optimum, pH 7.0) and with 0-2.0% NaCl (optimum, 0 % NaCl). 16S rRNA gene sequencing showed that strain YB22 was phylogenetically closest to the genus , and showed highest similarities to G4070 (96.7 %), ATCC 13253 (96.7 %), DSM 14571 (96.6 %), G0146 (96.5 %), G4122 (96.4 %) and R26 (96.2 %). Average amino acid identity values between strain YB22 and other taxa in the genus were all above the threshold range of genus determination. Average nucleotide identity and digital DNA-DNA hybridization values between strain YB22 and other phylogenetic relatives were all found to be below the threshold range for species determination. The respiratory quinone of strain YB22 was menaquinone 6 (MK-6) and the predominant cellular fatty acids were iso-C (47.8 %) and iso-C 3-OH (18.5 %). The major polar lipids were phosphatidylethanolamine, four unidentified aminolipids and three unidentified polar lipids. The phylogenetic analysis and physiological and biochemical data showed that strain YB22 should represent a novel species in the genus , for which the name sp. nov. is proposed. The type strain for this novel species is YB22 (=KCCM 43263 =JCM 32097 ).