Comparisons of nitrite accumulation, microbial behavior and nitrification kinetic in continuous stirred tank (ST) and plug flow (PF) moving bed biofilm reactors

Two types of continuous stirred tank moving bed biofilm reactors (ST-MBBR) and plug flow MBBR (PF-MBBR) were compared for nitrification. PF-MBBR showed strong shock resistance to temperature, and ammonium oxidation ratio (AOR) was 9.63% higher than that in the ST-MBBR, although the average biomass and biofilm thickness of ST-MBBR were 7.32–18.59%, 9.44–14.06% higher than those in the PF-MBBR. Meanwhile, a lower nitrite accumulation ratio (NAR) was observed (54.88%) in the PF-MBBR than the ST-MBBR (78.92%) due to different operation modes, and the divergence was demonstrated by the microbial quantitative analysis. Nitrification kinetics revealed that the temperature coefficient (θ) in the ST-MBBR (1.068) was much higher than that in the PF-MBBR (1.006–1.015), proving the contrasting nitrification performances caused by temperature shock. According to the Monod equation, the half-saturation coefficient (KN) in the ST-MBBR was 0.19 mg/L while it varied around 0.12–0.24 mg/L in the PF-MBBR, revealing various NH4+ affinity owing to different biofilm thickness and microbial composition. Finally, MBBR optimization related to operation mode, temperature, and free ammonium (FA) inhibition for nitrite accumulation was discussed. [Display omitted] •Ammonium oxidation ratio in the PF-MBBR was 9.63% higher than the ST-MBBR.•Nitrite accumulation ratio in the PF-MBBR was 24.04% lower than the ST-MBBR.•qPCR verified the correlation of nitrite accumulation and microbial population.•Kinetic parameters of θ, S, and KN were focused to reveal the nitrifying mechanism.•Free ammonium inhibition (>0.23 mg/L) for nitrite accumulation was discussed.