A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples

A rapid multiple immunofluorescence method for the simultaneous detection of CD20 and CD3 in canine and feline cytological samples

CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (...

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Veröffentlicht in:Journal of Veterinary Medical Science 2021, Vol.83(5), pp.832-836
Hauptverfasser: MATSUMOTO, Fumi, SHIMA-SAWA, Mariko, FURUSAWA, Yu, YAMATO, Osamu, YABUKI, Akira
Format: Artikel
Sprache:eng
Schlagworte:
CD20
CD20 antigen
CD3
CD3 antigen
Cellular biology
Cytology
immunocytochemistry
Immunofluorescence
immunophenotyping
Internal Medicine
Life Sciences & Biomedicine
Lymphocytes T
Lymphoma
Science & Technology
Veterinary Sciences
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Zusammenfassung:CD20 and CD3 are considered reliable markers for B and T cells, respectively. This study aimed to develop a rapid multiple immunofluorescence (RMIF) method for the detection of CD20 and CD3 on a single cytology slide. Air-dried smears were prepared using samples collected from dogs (n=26) and cats (n=6). Immunosignal detection using the newly developed method required 60 min. Clear immunosignals for CD20 and CD3 were detected in 24 of 26 samples in dogs and in all 6 cats. As the RMIF (CD20/CD3) method can detect markers of both B and T cells simultaneously on a single cytology smear, it would be an efficient tool for the immunophenotyping of canine and feline lymphoma samples.
ISSN:0916-7250
1347-7439
DOI:10.1292/jvms.20-0597