Urinary microbiota; Which non‐ınvasive urine collection method should we use?

Objective The aim of this study is to establish the optimal non‐invasive urine sample collection method for the microbiota studies. Methodology Twelve men with bladder carcinoma underwent first voided and midstream urine collection. Urine samples were analysed using V3‐V4 regions of bacterial 16s ribosomal RNAs. Bacterial groups with relative abundance above 1% were analysed in first voided urine and midstream urine samples at phylum, class, order and family level. At the genus level, all of the identified bacterial groups' relative abundances were analysed. The statistical significance (P