Enhanced osteogenic potential of unzipped carbon nanotubes for tissue engineering

Carbon nanotubes (CNTs) have attracted significant interest for various applications owing to their superior physicochemical properties. The unzipping of multi‐walled carbon nanotubes was accomplished by strong acid treatment. The solution of unzipped carbon nanotubes (u‐CNTs) was homogeneous and stable. The u‐CNTs were characterized by Fourier transform infrared spectroscopy, X‐ray diffraction, and thermogravimetric analysis. The dimensions and morphologies of the synthesized u‐CNTs were examined by transmission electron microscopy and scanning electron microscopy. The u‐CNTs exhibited increased zeta potential and diameter compared with pure CNTs. A decrease in the thermal stability was observed in the u‐CNTs compared with pure CNTs. The u‐CNTs exhibited better biocompatibility than pure CNTs in the presence of bone marrow‐derived mesenchymal stem cells, showing improved biocompatibility. The u‐CNT‐treated media generated lower amounts of reactive oxygen species than pure CNTs. Enhanced mineralization was observed in the u‐CNT‐treated groups compared with the pure CNTs and the control, indicating its better osteogenic potential. The upregulation of osteogenic‐associated gene markers in u‐CNT groups compared with pure CNTs confirms their superior osteogenic potential. Thus, u‐CNTs are potential candidates for tissue engineering applications, especially bone tissue.