TNF-α inhibition, antioxidant effects and chemical analysis of extracts and fraction from Brazilian guaraná seed powder
•Fractionation of seed powder guaraná result a fraction highly active.•Identification of type-A procyanidin trimer by LC-ESI-MS.•High inhibition of TNF-α release in fraction from guaraná.•Strong antioxidant potential was verified in guaraná extracts and fraction. Paullinia cupana Kunth., commonly na...
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Veröffentlicht in: | Food chemistry 2021-09, Vol.355, p.129563-129563, Article 129563 |
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Sprache: | eng |
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Zusammenfassung: | •Fractionation of seed powder guaraná result a fraction highly active.•Identification of type-A procyanidin trimer by LC-ESI-MS.•High inhibition of TNF-α release in fraction from guaraná.•Strong antioxidant potential was verified in guaraná extracts and fraction.
Paullinia cupana Kunth., commonly named Guaraná, is a plant from Brazil used as stimulant. The aim of this study was to evaluate the potential of extracts and tannins-rich and methylxanthines-free fraction from guaraná in the anti-inflammatory and antioxidant effect in vitro. Extract 1 obtained good yields of tannins and methylxanthines and was used to identify a type-A procyanidin trimer by LC-ESI-MS. Fraction 4 was rich in tannins and absent of methylxanthines. The extracts and fraction exhibited strong capacity for scavenging DPPH radical with IC50 between 5.88 and 42.75-µg/mL and inhibited TNF-α release by LPS-activated THP-1 cells when compared with control cells and did not present toxicity to THP-1 cells. The fraction 4, rich in tannins, was highly active, with IC50 5.88 µg/mL by DPPH method and inhibited TNF-α release in 83.50% at 90 µg/mL. These results reinforced potential anti-inflammatory of guaraná and data for new therapeutic approaches. |
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ISSN: | 0308-8146 1873-7072 |
DOI: | 10.1016/j.foodchem.2021.129563 |