Bio-typing of Mycobacterium avium subspecies paratuberculosis isolates recovered from the Himalayan sheep and goats
Information on bio-type profile of Mycobacterium avium subspecies paratubeculosis (MAP) in sheep flocks and goat herds of Himalayan region is not reported earlier. The aim of our study was to determine the bio-type of MAP infecting livestock of this region. A total of 71 faecal samples (sheep—57, go...
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Veröffentlicht in: | Tropical animal health and production 2021-06, Vol.53 (2), p.237-237, Article 237 |
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Zusammenfassung: | Information on bio-type profile of
Mycobacterium avium
subspecies
paratubeculosis
(MAP) in sheep flocks and goat herds of Himalayan region is not reported earlier. The aim of our study was to determine the bio-type of MAP infecting livestock of this region. A total of 71 faecal samples (sheep—57, goats—14) were screened by Ziehl–Neelsen (ZN) staining and IS
900
PCR, and then processed for culture on Herrold’s egg yolk medium (HEYM) having mycobactin J (MJ). Out of 71 faecal samples, MAP colonies were seen only in four samples (sheep—3 and goat—1). Isolates were confirmed as MAP on the basis of slow growth, acid fastness, MJ dependency, IS
900
and IS
1311
PCR. All the IS
900
and IS
1311
PCR positive samples were bio-typed by IS
1311
PCR-REA (restriction endonuclease analysis), which confirmed all four isolates as ‘bison type.’ In IS
1311
based phylogeny of MAP isolates by ClustalW method of the MegAlign program of DNASTAR Lasergene software, the four sequences of MAP isolates (NCBI sequence nos. MH988763, MH988765, MH988766 and MH988764) did not show any distinct clustering/grouping pattern. However, these four isolates showed a bit of closeness to the MAP sequences (KC990353.1 and KC990352.1) of ‘bison type’ isolated from wood bison in Canada. In conclusion, this is the first report on isolation and bio-type profile of MAP infecting sheep and goats of Himalayan region. Study will help in devising prevention and control strategies against spread of MAP infection in livestock population of Himalayan region. |
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ISSN: | 0049-4747 1573-7438 |
DOI: | 10.1007/s11250-021-02682-7 |