Survey of diagnostic and typing capacity for enterovirus infection in Italy and identification of two echovirus 30 outbreaks
•A survey on laboratories diagnosis capacity for Enterovirus infections has been carried out in Italy.•An homogenous laboratory capacity for EV diagnosis was evidenced, with 21,000 diagnostic tests reported in 2018.•Real time RT-PCR is the method of choice as routine diagnostic assay.•Two outbreaks...
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Veröffentlicht in: | Journal of clinical virology 2021-04, Vol.137, p.104763-104763, Article 104763 |
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Zusammenfassung: | •A survey on laboratories diagnosis capacity for Enterovirus infections has been carried out in Italy.•An homogenous laboratory capacity for EV diagnosis was evidenced, with 21,000 diagnostic tests reported in 2018.•Real time RT-PCR is the method of choice as routine diagnostic assay.•Two outbreaks of Echovirus 30 confirmed by molecular analyses, occurred in 2019.•The study underlines the need to monitor the circulation of NPEVs to ascertain the real burden of the disease in the country
Enterovirus infections can cause a variety of illnesses, ranging from asymptomatic infections to severe illness and death.
To support polio eradication activities, in February 2019, the WHO Regional Reference Laboratory for polio in Italy, at the National Institute of Public Health (Istituto Superiore di Sanità), promoted an investigation on non-polio enterovirus laboratory capacity, with the support of the Italian Ministry of Health. The aim was to collect data on the assays used routinely for diagnostic purposes and to characterize enterovirus outbreaks strains by sequence analysis of the Viral Protein 1 region.
A questionnaire was administered to public health laboratories through all Italian Regions for 2018 and subsequently, an electronic form for lab-confirmed enterovirus infection reported from February 2019 to January 2020, including patients clinical characteristics, and laboratory data was distributed through 25 laboratories participating the survey.
Overall, a homogenous laboratory capacity for enterovirus infection diagnosis was found and 21,000 diagnostic tests were retrospectively reported in 2018. Then, in 2019, two outbreaks of Echovirus 30 were identified and confirmed by molecular analyses.
These results underline the need monitor the circulation of non-polio enterovirus to ascertain the real burden of the disease in the country. |
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ISSN: | 1386-6532 1873-5967 |
DOI: | 10.1016/j.jcv.2021.104763 |