Effect of an auxin biosynthesis inhibitor, p-phenoxyphenyl boronic acid, on auxin biosynthesis and development in rice

p-Phenoxyphenyl boronic acid (PPBo) is a specific inhibitor of auxin biosynthesis in Arabidopsis. We examined the inhibitory activity of PPBo in rice. The activity of OsYUCCA, a key enzyme for auxin biosynthesis, was inhibited by PPBo in vitro. The endogenous indole-3-acetic acid (IAA) level and the expression levels of auxin-response genes were significantly reduced in PPBo-treated rice seedlings, which showed typical auxin-deficiency phenotypes. Seminal root growth was promoted by 1 mu M PPBo, which was reversed by co-treatment of IAA and PPBo. By contrast, the inhibition of root growth by 10 mu M PPBo was not recovered by IAA. The root meristem morphology and cell division were restored by IAA at 60 mu M, but that concentration may be too high to support root growth. In conclusion, PPBo is an inhibitor of auxin biosynthesis that targets YUCCA in rice.