An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens

An efficient heterologous Escherichia coli-based expression system for lectin production from Pleurocybella porrigens

In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli-based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to o...

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Veröffentlicht in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2021-02, Vol.85 (3), p.630-633
Hauptverfasser: Suzuki, Tomohiro, Nakamura, Luna, Inayoshi, Satomi, Tezuka, Yuki, Ono, Akiko, Choi, Jae-Hoon, Dohra, Hideo, Sasanami, Tomohiro, Hirai, Hirofumi, Kawagishi, Hirokazu
Format: Artikel
Sprache:eng
Schlagworte:
Biochemistry & Molecular Biology
Biotechnology & Applied Microbiology
Chemistry
Chemistry, Applied
Food Science & Technology
Life Sciences & Biomedicine
Physical Sciences
Science & Technology
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Zusammenfassung:In this study, we report a more efficient heterologous expression of lectin from Pleurocybella porrigens (PPL) using an Escherichia coli-based expression system. The yield (9.3 mg/L culture broth) of recombinant PPL (rPPL) using this expression system was increased approximately 9-fold compared to our previous study. The rPPL obtained in this study exhibited the same biochemical properties as the native PPL.
ISSN:0916-8451
1347-6947
1347-6947
DOI:10.1093/bbb/zbaa058