Transposon-Associated CRISPR-Cas System: A Powerful DNA Insertion Tool

Transposon-Associated CRISPR-Cas System: A Powerful DNA Insertion Tool

The transposon-associated CRISPR-Cas system is emerging as a powerful tool for targeted genetic manipulation. Using this system, targeted genomic knockins and knockouts can be achieved, independently of the host-cell repair machinery, thereby significantly improving our ability to perform genome edi...

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Veröffentlicht in:Trends in microbiology (Regular ed.) 2021-07, Vol.29 (7), p.565-568
Hauptverfasser: Ma, Wang, Xu, Ying-Shuang, Sun, Xiao-Man, Huang, He
Format: Artikel
Sprache:eng
Schlagworte:
CRISPR
Deoxyribonucleic acid
DNA
DNA insertion
Genome editing
Insertion
microbial community genome editing
targeted genome editing
transposable elements
transposon-associated CRISPR-Cas system
Transposons
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Zusammenfassung:The transposon-associated CRISPR-Cas system is emerging as a powerful tool for targeted genetic manipulation. Using this system, targeted genomic knockins and knockouts can be achieved, independently of the host-cell repair machinery, thereby significantly improving our ability to perform genome editing in a wider variety of cell types.
ISSN:0966-842X
1878-4380
DOI:10.1016/j.tim.2021.01.017