Novel strategy for enhancing acetic and formic acids generation in acidogenesis of anaerobic digestion via targeted adjusting environmental niches

•pH, ORP and C/N ratio were optimized for enhancing acetate and formate generation•The maximal VFAs yield was achieved at pH of 6.0 or ORP of -350 mV•Acetic and formic acids were the highest at pH of 7.0 or ORP of -450mV•The abundances of microorganisms producing acetate and formate increased at pH 7.0•Optimized parameters were verified in CSTR for producing acetic and formic acids Optimization of acetic acid and formic acid production efficient methanogenesis is always the research hot spot in anaerobic digestion. It is a promising approach to adjust the operation parameters to influence the functional microorganisms for better acetic acid and formic acid production in acidogenesis. Herein, the effects of pH, oxidation-reduction potential (ORP) and carbon–nitrogen (C/N) ratio were determined in batch experiments to probe acetic and formic acids production, and were further verified in continuous stirred tank reactor (CSTR). The results revealed that the content of volatile fatty acids (VFAs) reached to maximum at pH 6.0 or ORP -350 mV, while the production of acetic and formic acids was the highest at pH 7.0 or ORP -450 mV in 9 h fermentation. Also, fermentation products dominated by acetic and formic acids were adjusted in the CSTR under the operating conditions of pH 7.0 and ORP -450 mV. Microbiological analysis from batch test showed that fermentation at pH value of 7.0 enriched the diversity of microorganism, and provided a niche for microbes (Petrimonas, norank_f__Synergistaceae, vadinBC27_wastewater-sludge_group, and Trichococcus) to produce acetic and formic acids. Correspondingly, 78.70% of the carbon was converted to acetic and formic acids in pH 7.0. This study provides a promising strategy for the targeted regulation of acetic and formic acids production in acidogenesis of anaerobic digestion. [Display omitted]