Characterization of two newly emerged torque teno sus virus isolates from a large-scale pig farm in China, in 2018

Torque teno sus virus (TTSuV) infection is common in China's pig herd. Although of uncertain pathogenicity, TTSuVs have been reported as a worsening factor of other porcine diseases, including porcine circovirus associated disease (PCVAD), porcine respiratory diseases complex (PRDC) or porcine dermatitis and nephropathy syndrome (PDNS). To better understand the genetic diversity in TTSuVs, the complete genomes of two newly emerged isolates, referred to as HeN1-A9 and HeN1-A11, collected from pig samples at a large-scale pig farm in China, were analyzed. Phylogenetic relationships of TTSuV sequences separated TTSuV1 and TTSuVk2a groups and divided TTSuV1 into two major subtypes, including TTSuV1a and TTSuV1b; HeN1-A9 and HeN1-A11 strains classified into the TTSuV1a subtype. Recombination analysis demonstrated HeN1-A9 and HeN1-A11 were generated via recombination in the overlapping ORF1/ORF3 region of TTSuV1a genome, which we report for the first time. Furthermore, we found that HeN1-A9 could be replicated in cultured MARC-145 cells for 18 passages. Our findings may be useful for elucidating the characteristics and epidemic status of TTSuVs in China. •We found sub-healthy nursery piglets co-infected with TTSuVs, PRRSV and PCV2.•The complete genomes of two newly emerged TTSuV1 strains, HeN1-A9 and HeN1-A11, were sequenced and analyzed.•HeN1-A9 and HeN1-A11 strains were generated via recombination, and the recombination region was novel.•The HeN1-A9 strain could be replicated in in vitro cultured MARC-145 cells for 18 passages.