Protein and phytohormone profiles of Mahanarva spectabilis salivary glands infesting different forages

Given the importance of pastures for feeding cattle, the study of factors that affect their productivity is essential to get plant material of higher nutritional quality. Thus, the study of insect–plant interaction is important for the development of control strategies. Pasture spittlebugs affect forage grasses causing severe damage. We tested hormone and protein profiles differentially expressed in the salivary glands of Mahanarva spectabilis when fed with different pasture genotypes. The LC/MS approaches combined with bioinformatics tools were used to identify the mains biological processes in the salivary glands. The grouping revealed a greater number of proteins involved in biological processes of metabolic synthesis, biotic/abiotic stress, and ion transport across the membrane. The proteomic profiles were altered when insects were fed with different grasses. We also detected phytohormones in the salivary glands involved in the modulation of defense responses in host plants. These results allowed the analysis of important biological processes such as cell homeostasis, stress proteins, nucleic acid metabolism, regulation of muscle contraction, and transport and export of biomolecules. This represents an important advance in the understanding of the plant–pest interaction and can contribute to the choice of target elicitors, which allow effective strategies in the control of pasture spittlebugs. Molecular profiling of the interaction between Mahanarva spectabilis and grasses. Research Highlights Molecular interaction between Mahanarva spectabilis and grasses. Proteomic profile of M. spectabilis changed when fed by different genotypes. Phytohormones in the salivary glands involved in the modulation of defense responses in host plants.