H2S-mediated aerobic exercise antagonizes the hippocampal inflammatory response in CUMS-depressed mice
•The mechanism of H2S involved in mediating aerobic exercise to antagonize depressive hippocampal inflammation is proposed.•This mechanism reflects that exercise can up-regulate the expression of H2S and its related factors while down-regulating inflammatory factors.•H2S enhancement can promote the...
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Veröffentlicht in: | Journal of affective disorders 2021-03, Vol.283, p.410-419 |
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Sprache: | eng |
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Zusammenfassung: | •The mechanism of H2S involved in mediating aerobic exercise to antagonize depressive hippocampal inflammation is proposed.•This mechanism reflects that exercise can up-regulate the expression of H2S and its related factors while down-regulating inflammatory factors.•H2S enhancement can promote the effect of aerobic exercise against depressive hippocampal inflammation.•This mechanism verifies that endogenous H2S-mediated aerobic exercise antagonizes the inflammatory process of depression.
This thesis was to investigate the protective effect and mechanism of H2S-mediated aerobic exercise on the antagonism of the hippocampus inflammatory response in CUMS-depressed mice.
Seventy C57BL/6 mice were randomly divided into control group (CG), model control group (MG), model exercise group (ME), H2S enhanced group (HG) and H2S enhanced and exercise group (HE). All mice except CG underwent a 28-day CUMS depression model. ME and HE received moderate-intensity aerobic treadmill training for 8 weeks. They were randomly selected for Nissl staining, Immunofluorescence, methylene blue colorimetric assay, and ELISA. The levels of IL-10 and TNF-ɑ were detected by qRT-PCR, and the expression levels of CBS and inflammatory-related factors in the hippocampus were detected.
Compared with CG, the number of erections, modifications, and crossing grids in MG mice were significantly reduced, the time of forced swimming and forced tail suspension was significantly prolonged, the positive rate of 5-HT decreased, and the symptoms of depression were obvious. The positive rate of CD45+ increased, the inflammatory response was obvious, and the content of H2S and the expression of biosynthetic enzyme CBS decreased. Aerobic exercise and H2S-enhanced mice improved depressive symptoms, decreased proinflammatory factors, increased anti-inflammatory factors, increased H2S content, increased CBS expression, and increased H2S.
H2S may participate in aerobic exercise to antagonize the inflammatory process of the hippocampus in CUMS-depressed mice by reducing the release of inflammatory response factors and hippocampus nerve injury factors, and effectively alleviate inflammatory injury in the hippocampus of depressed mice. |
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ISSN: | 0165-0327 1573-2517 |
DOI: | 10.1016/j.jad.2021.02.005 |