Review: Microtubules monitor calcium and reactive oxygen species signatures in signal transduction

•Ca2+-MT-ROS crosstalk is required for signal propagation.•Microtubules dynamicity mediates calcium and ROS signatures.•Stimulus type conducts the prioritization of ROS or Ca2+ signalling.•Ca2+ influx activates ROS to propagate signal transduction. Signal transductions require calcium (Ca2+) or reactive oxygen species (ROS) signatures, which act as chemical and electrical signals in response to various biotic and abiotic stresses. Calcium as an ion or second messenger affects the membrane potential and microtubules (MTs) dynamicity, while MTs can modulate auto-propagating waves of calcium and ROS signatures in collaboration with ion channels depending on the stimulus type. Thus, in the current review, we highlight advances in research focused on the relationship between dynamic MTs and calcium and ROS signatures in short-distance transmission. The challenges of Ca2+-MTs-ROS crosstalk in cold sensing are addressed, which could suggest the prioritization of ROS or Ca2+ in signalling.