Nocardia bovistercoris sp. nov., an actinobacterium isolated from cow dung

A novel actinobacterium, designated strain NEAU-351 , was isolated from cow dung collected from Shangzhi, Heilongjiang Province, northeast PR China and characterized using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain NEAU-351 belonged to the genus , with the highest similarity (98.96 %) to DSM 44801 and less than 98.0 % identity with other type strains of the genus . The polar lipids consisted of diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylinositol. The major menaquinone was observed to contain MK-8(H , ω-cycl) (78.2 %). The fatty acid profile mainly consisted of C , C 9 and 10-methyl C . Mycolic acids were present. The genomic DNA G+C content of strain NEAU-351 was 68.1 mol%. In addition, the average nucleotide identity values between strain NEAU-351 and its reference strains, DSM 44801 and NBRC 108935 , were found to be 81.4 and 82.9 %, respectively, and the level of digital DNA-DNA hybridization between them were 24.8 % (22.5-27.3 %) and 26.3 % (24-28.8 %), respectively. Here we report on the taxonomic characterization and classification of the isolate and propose that strain NEAU-351 represents a new species of the genus , for which the name is proposed. The type strain is NEAU-351 (=CCTCC AA 2019090 =DSM 110681 ).